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Schroeder CA Jr, Lee HT, Shah PM, Babu SC, Thompson CI, Belloni
FL. Reconditioning with ischemia or adenosine protects skeletal
muscle from ischemic tissue reperfusion injury. J Surg Res. 1996;Jun;63(1):29-34.
Department of Surgery, Westchester County Medical Center, New
York Medical College, Valhalla 10595, USA.
Prolonged tissue ischemia and subsequent reperfusion results in significant
tissue injury due to the ischemic-reperfusion (IR) syndrome. Ischemic
preconditioning (IPC) or adenosine (ADO) pretreatment are known to protect
IR injury in cardiac muscle. Our aim was to determine whether IPC or
ADO pretreatment attenuates and protects against ischemic tissue reperfusion
injury in skeletal muscle. Rats were anesthetized and global hindlimb
ischemia was induced by 60 min of suprarenal aortic clamping followed
by 30 min of reperfusion period. The degree of skeletal muscle dysfunction
was determined by decreases in maximum contractile force, and adenosine
triphosphate (ATP) and creatine phosphate (CP) levels of extensor digitorum
longus (EDL) muscle. The distal tendon of the EDL was attached to a force
transducer for maximum isometric force measurement. Samples were taken
from the EDL for measurement of ATP and CP levels. The following were
protective protocols prior to the IR challenge: (1) four consecutive
5-min periods of ischemia separated by 5-min reperfusion periods (PC/I)
or (2) i.v. adenosine infusion (350 microg/kg/min x 10 min, PC/A). Our
data suggest that pretreatment with brief periods of ischemia or systemic
ADO infusion attenuates ischemic tissue reperfusion injury in skeletal
muscle.
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Abstracts